Table S1 ) and k-means clustering of cell populations according to the reporter expression level ( k = 3 green - g s c m 1 -, light blue - g s c m 2 - and magenta - g s c m 3 - for the mean expression level in each cluster). (B) Average time course of nuclear staining intensity along cell trajectories for each cluster: n u c m 1 , n u c m 2 and n u c m 3 . (C) Average value of the gsc reporter expression along each cluster’s cell trajectories, normalized by nuclear staining intensity ( g s c N m 1 , g s c N m 2 and g s c N m 3 ). (D) Reduced-order maps of gsc reporter expression showing three domains and their spatiotemporal organization (green - g s c m 1 -, light blue - g s c m 2 - and magenta - g s c m 3 , time points 8 and 10 hpf indicated top right of each panel). Cells at 8 hpf are color-labeled according to the cluster they belong to and the color is propagated along the cell lineage. (E) Reduced-order biomechanical map showing four domains and their spatiotemporal organization similar to wt1 in
(time points 8 and 10 hpf indicated top right of each panel, t i n i = 8 hpf). Scale bar 50 μ m . " width="100%" height="100%">
Journal: iScience
Article Title: Unsupervised spatiotemporal classification of deformation patterns of embryonic tissues matches their fate map
doi: 10.1016/j.isci.2025.111753
Figure Lengend Snippet: Reduced-order models of tissue deformation and gsc reporter expression yield corresponding maps (A – E) wt6 embryo from the transgenic line Tg(gsc:EGFP) imaged from 8 to 13 hpf (A) Quantification of gsc reporter expression at the single cell level ( , Table S1 ) and k-means clustering of cell populations according to the reporter expression level ( k = 3 green - g s c m 1 -, light blue - g s c m 2 - and magenta - g s c m 3 - for the mean expression level in each cluster). (B) Average time course of nuclear staining intensity along cell trajectories for each cluster: n u c m 1 , n u c m 2 and n u c m 3 . (C) Average value of the gsc reporter expression along each cluster’s cell trajectories, normalized by nuclear staining intensity ( g s c N m 1 , g s c N m 2 and g s c N m 3 ). (D) Reduced-order maps of gsc reporter expression showing three domains and their spatiotemporal organization (green - g s c m 1 -, light blue - g s c m 2 - and magenta - g s c m 3 , time points 8 and 10 hpf indicated top right of each panel). Cells at 8 hpf are color-labeled according to the cluster they belong to and the color is propagated along the cell lineage. (E) Reduced-order biomechanical map showing four domains and their spatiotemporal organization similar to wt1 in Figure 5 (time points 8 and 10 hpf indicated top right of each panel, t i n i = 8 hpf). Scale bar 50 μ m .
Article Snippet: Digital embryo zebrafish wild-type + gsc wt6 , CNRS-BioEmergences, this paper, Mendeley Data , Mendeley Data: https://doi.org/10.17632/58rz299z74.1.
Techniques: Expressing, Transgenic Assay, Staining, Labeling